TABLE 1 |.
FST qPCR assays used and their performance parameters.
| Assay name | Target | References | F and R primer conc. (μM) | Probe conc. (μM) | R2 | Eff. (%)a | LLOQb |
|---|---|---|---|---|---|---|---|
| DG3 | Canine | (Green et al., 2014b) | 1.4 | 0.1 | 0.994 | 97.2 | 10 |
| HF183 | Human | (Bernhard and Field, 2000; Green et al., 2014a) | 1 | 0.08 | 0.994 | 98.8 | 10 |
| Modified GFD | Avian | (Green et al., 2012) | 1 | 0.1 | 0.993 | 97.4 | 10 |
| Rum2Bac | Ruminant | (Mieszkin et al., 2010) | 0.3 | 0.1 | 0.991 | 99.9 | 10 |
| CG4 | C. elegans internal control | (Kirtane et al., 2019) | 1.4 | 0.1 | 0.943 | 99.9 | 10 |
a
Calculated using %Eff = (10(−1/slope))-1, where slope is the assay-specific slope of 4–6 pooled standard curves constructed using synthetic gBlocks® (IDT).
b
Assay lower limits of quantification (gBlock® copies/reaction).