Table 1. Advantages of in utero electroporation.

Advantages	Details	References
High efficiency, low cytotoxicity	No significant increase in cell death has been detected after electroporation	Saito (2006), Mizutani and Saito (2005)
Simple and quick procedure	In vivo electroporation for gene transfer into more than 10 embryos can be carried out within 30 min	Saito (2006)
Multiple gene targeting	Multiple genes on different plasmids can be simultaneously transfected into the same cells	Saito and Nakatsuji (2001)
Region and time-specific gene targeting	Only the side of the ventricle that is close to the anode is transfected	Saito and Nakatsuji (2001), Saba et al. (2003), Cwetsch et al. (2018)