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. 2020 Apr 30;9(1):1757209. doi: 10.1080/20013078.2020.1757209

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Trypsin treatment of EVs derived from monocytic lymphoma U937 cells and immortalized human dermal microvascular endothelial HMEC-1 cells. Western blotting shows that trypsin-resistant vesicular proteins (CD81, GAPDH, and actin) are not affected but only trypsin-sensitive vesicular proteins (ICAM1, calnexin, and RPL14) are digested by trypsin in U937 EVs (a) and HMEC-1 EVs (b) as observed in SW480 EVs. Additional biological replicate of Western blotting for U937 EVs are shown in Figure S3. Note that CD81 is not expressed in U937 cells [24].