Figure 4.

Schistosomula EVs are internalised via interactions with CLR DC-SIGN.

PKH26 fluorescence of CD1a⁺ cells with indicated pre-incubations (x-axis). The geoMFI (geometric mean fluorescent intensity) is shown relative to cells in medium only (a). MoDC from three to six donors. Representative histograms of two donors from same data plotted in a (b). Confocal microscopy visualisation of EV uptake and inhibition of EV uptake when DC-SIGN was blocked (c). Images show maximum projections of recorded z-stacks. PKH26-labelled EVs are stained in red and the nuclei in blue (Hoechst). Scale bar is 10 µm. Selected images are representative for three donors. Mean±SEM ***p < 0.001, using repeated measures ANOVA with Tukey’s Multiple Comparison Test. MR, mannose receptor