(A) DNA test results from 10 different cooked conditions. Lanes 1–3 are quantitative standards, K562 (50, 25, 12.5 ng); lane 4 is a negative control; lanes 5–14 are tomato seeds that were boiled for 1, 3, 5, 10, 15, 20, 30 s and 1, 3, 5 min; lanes 15–16 are standard size markers 1 Kb and 100 bp ladder (New England Biolabs, Beverly, MA, USA). (B) DNA test results from 10 different cooked conditions. Lanes 1–3 are quantitative standards, K562 (50, 25, 12.5 ng); lanes 4–9 are tomato seeds that were boiled for 10, 20, 30 min and 1, 2, 3 h; lane 10 is a pan-fried tomato seed that was cooked above 100 °C for 3 min; lanes 11–13 are tomato seeds that were cooked at 218 °C for 10, 20 or 30 min); lane 14 is DNA from fresh seed; lanes 15–16 are standard size markers 1 Kb and 100 bp ladder (New England Biolabs). (C) Photomicrographs of the seed surface morphology from seeds cooked at 10 different cooking times (from 30 s to 3 h) at 100 °C and one fresh tomato seed.