Figure 5.

Confirmation of the cytotoxicity enhancing activity of DI3A. (A) H1299-fLuc + cells were co-cultured with NK cells in the presence or absence of 10 nM DI3A at the effector to target (E: T) ratio of 1:1. Lysis was assessed using biophotonic cytotoxicity assay after 24 h incubation. (B) H1299 or H1975 cells were co-cultured with NK cells in the presence or absence of 10 nM DI3A at the effector to target (E: T) ratio of 1:1. Lysis was assessed using calcein release assay after 4 h incubation. 100 U of IL-2 was employed as a positive control. Data shown represent means ± SD, *p < 0.05; **p < 0.01; ***p < 0.001.