Table 2. Primers used in this study.
| Primer | Sequence (5′–3′)a | Application |
|---|---|---|
| rsp-up-F-KpnI | GCGggtaccAAGGTGTTAATTCATTGATG | rsp deletion |
| rsp-up-R | ATTTCTCTCTCCTGCCTAAT | rsp deletion |
| rsp-down-F | ATTAGGCAGGAGAGAGAAATATACTAACAGTCCTCTTGTG | rsp deletion |
| rsp-down-R-SalI | ACGCgtcgacAACTAATACCAAGACCAAAA | rsp deletion |
| Crsp-F-KpnI | GCGggtaccTTACGGCAATAACTAGATGG | rsp chromosomal complementation |
| Crsp-R-SalI | ACGCgtcgacTGCAGTATTTATATTACATA | rsp chromosomal complementation |
| agr-up-F-KpnI | GCGggtaccAAGAGGTTGAACAAGCATTT | agr deletion |
| agr-up-R | GCAGCGATGGATTTTATTTT | agr deletion |
| agr-down-F | AAAATAAAATCCATCGCTGCGATGAATAATTAATTACTTTCAT | agr deletion |
| agr-down-R-SalI | ACGCgtcgacGTCATTGGAACTAATAGCAC | agr deletion |
| pLI50-rsp-F-SacI | TCCgagctcTATTGTTTTTTGAAATACATAGG | rsp overexpression |
| pLI50-rsp-R-KpnI | CGGggtaccTTGAATTGCTTGTGAGTTAC | rsp overexpression |
| pRMC2-rsp-F-KpnI | CGGggtaccATTAGGCAGGAGAGAGAAAT | rsp induced expression |
| pRMC2-rsp-R-EcoRI | CCGgaattcAGGACTGTTAGTATTTAGCT | rsp induced expression |
| RT-rsp-F | ACGATATTCACGATATTGAGATT | qRT-PCR |
| RT-rsp-R | TCTTGCTTGTCTTATAGTCTTG | qRT-PCR |
| RT-agrA-F | TATGGCGATTGACGACAA | qRT-PCR |
| RT-agrA-R | GCAGTAATTCAGTGTATGTTCA | qRT-PCR |
| RT-psmα-F | GTATCATCGCTGGCATCA | qRT-PCR |
| RT-psmα-R | AAGACCTCCTTTGTTTGTTATG | qRT-PCR |
| RT-psmβ-F | TGGACTAGCAGAAGCAATC | qRT-PCR |
| RT-psmβ-R | TAGTAAACCCACACCGTTAG | qRT-PCR |
| RT-hla-F | GGTATATGGCAATCAACTT | qRT-PCR |
| RT-hla-R | CTCGTTCGTATATTACATCTAT | qRT-PCR |
| RT-hu-F | AAAAAGAAGCTGGTTCAGCAGTAG | qRT-PCR |
| RT-hu-R | TTTACGTGCAGCACGTTCAC | qRT-PCR |
| Prsp-F-EcoRI | CCGgaattcGTATTGTTTTTTGAAATACATAG | pOSrsp |
| Prsp-R-BamHI | CGCggatccGGTTTAAGTTGGCATGTCATAT | pOSrsp |
| Phla-F-EcoRI | CCGgaattcTTCAACTTTGACTAACCCTC | pOShla |
| Phla-R-BamHI | CGCggatccGGGACTATACGTGTTTTCATTT | pOShla |
| Ppsmα-F-EcoRI | CCGgaattcTCTGTTCAATTCATCTTCATA | pOSpsmα |
| Ppsmα-R-BamHI | CGCggatccGGGCCAGCGATGATACCCATTAAG | pOSpsmα |
| Ppsmβ-F-EcoRI | CCGgaattcGGCAACTTAATTGTGTTAAA | pOSpsmβ |
| Ppsmβ-R-BamHI | CGCggatccGGGTTAAATAAACCTTCCATTG | pOSpsmβ |
| E-rsp-F-BamHI | CGCggatccGATGACATGCCAACTTAAAAT | Rsp expression |
| E-rsp-R-EcoRI | CCGgaattcTTAGCTTGGTTTAAAGCAAA | Rsp expression |
| probe-prsp-F319 | GTATTGTTTTTTGAAATACATAG | EMSA |
| probe-prsp-F139 | TTCACTATTACCTTTTCAAAA | EMSA |
| probe-prsp-F89 | TGATTTTAAAAATTTCCATG | EMSA |
| probe-prsp-Biotin-R | TTTAAGTTGGCATGTCATAT | EMSA |
| probe-phla-F310 | TTAGCTATGTCTTTTCCTTG | EMSA |
| probe-phla-F239 | CCTTTCAAATTTTAAATAAA | EMSA |
| probe-phla-F189 | CATCATCACTCAGTAATTTA | EMSA |
| probe-phla-F110 | TGCAATATTCTAAATTGACA | EMSA |
| probe-phla-Biotin-R | TAGTGTTGTTGTTACTGAG | EMSA |
| probe-ppsmα-F326 | TCTGTTCAATTCATCTTCATA | EMSA |
| probe-ppsmα-F166 | ACCTGTACAGTTAGGCAGTA | EMSA |
| probe-ppsmα-F85 | AAAATCAATTACGCACAAGA | EMSA |
| probe-ppsmα-Biotin-R | GCCAGCGATGATACCCATTAAG | EMSA |
| probe-ppsmβ-F253 | ACTTAAATACGAATTCAGGCAACT | EMSA |
| probe-ppsmβ-F162 | CATTATAAGATGTTGTGCGG | EMSA |
| probe-ppsmβ-F139 | AACAAACTAATTGCATCAAA | EMSA |
| probe-ppsmβ-F89 | AAGCGAATAACATTTCGGTG | EMSA |
| probe-ppsmβ-Biotin-R | AACCTTCCATTGAAAACACTCC | EMSA |
aLowercase letters indicate restriction sites. Letters in bold indicate complementary sequences used for overlap pcr ligation.