Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 25;42(1):350–357. doi: 10.1080/0886022X.2020.1751658

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — PTEN is the direct target of miR-181 in vitro. (A) The base-pairing sites of miR-181 and the 3′ UTR of PTEN mRNA, as predicted by bioinformatics software. (B) The miR-181 expression measured by quantitative real-time PCR (normalized to U6) 48 h after transfection with miR-181 mimic or NC in 293T cells. (C) The PTEN protein levels measured by western blotting 48 h after transfection with miR-181 mimic or NC in 293T cells. GAPDH was used as the reference gene. (D) A quantitative analysis of protein levels normalized to GAPDH was by Image J software. Statistical significance analysis was by the two-tailed Student’s t-test.