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. Author manuscript; available in PMC: 2020 May 21.
Published in final edited form as: Adv Funct Mater. 2018 Mar 25;28(19):1800228. doi: 10.1002/adfm.201800228

Figure 4.

Figure 4.

hMSC alignment on the biosilica micropatterned and control hydrogels. (a, b, c) hMSCs aligned to the biosilica micropatterns on the silk hydrogels; (d, e, f) hMSCs on the unpatterned control silk hydrogels demonstrated typical morphology. Confocal micrographs of hMSCs on the silk hydrogels stained for F-actin (green, a, d), counterstained for nuclei and silk (blue, b, e) and overlay images (c, f). (g) Fluorescence images of hMSCs stained for F-actin (green) on the micropatterned “Tufts” logo. (h) Partial enlargement of Figure g. (i) Zoomed view (left) and SEM image (right) of hMSCs on the biosilica micropatterns. (j) Graphical representation of cellular alignment quantified via ImageJ software and an OrientationJ plug-in. (k) Coherency coefficients indicating cellular distribution on the micropatterned and control hydrogels. Scale bars, a-f, 200 μm; g, 2 mm; h, 700 μm; i, 50 μm (left), 10 μm (right).