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. 2020 Apr 28;11:350. doi: 10.3389/fphar.2020.00350

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Copyright © 2020 Zeng, Zhang, Wang, Cheng, Zhang and Zhou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of donepezil, memantine, melatonin, and LW on lymphocyte subsets of splenocyte in old SAMR1 mice. 1× 10⁶ spleen cells isolated from mouse (n = 6) spleen were harvested, (A) CD19⁺ B cells, (B) CD3⁺ CD4⁺ T cells, (C) CD3⁺ CD8⁺ T cells, (D) CD4⁺ CD25⁺ T cells, (E) CD4⁺ CD28⁺ T cells, (F) CD8⁺ CD28⁺ T cells quantified by flow cytometry. *P < 0.05, **P < 0.01, ***P < 0.001 vs control, one-way ANOVA and Dunnett test. mean ± S.D., n=6~9(male: n = 2~4, female: n = 4~6). (G) The scatter plot of lymphocyte subsets.