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. 2020 May 21;15(5):e0225356. doi: 10.1371/journal.pone.0225356

Fig 3. MiR-148a reduces cellular LDL uptake by specifically modulating (P)RR expression.

Fig 3

HepG2 and Huh7 cells were transfected with control miRNA or miR-148a, together with control inhibitor (CTI) or miR-148a inhibitor (miRI) for 48 hours. Cells were cultured with serum containing medium. A. (P)RR and LDLR mRNA levels were determined by quantitative PCR. Results are from 4 independent experiments in triplicates. N = 12; **: p<0.01; ***: p<0.001. B & C. HepG2 (B) and Huh7 cells (C) were treated as above, and total cell lysates were immunoblotted as indicated. Representative blots of 4 independent experiments in duplicate were shown. (P)RR and LDLR protein abundance were quantified. N = 8. **: p<0.01; ***: p<0.001. D & E. HepG2 and Huh7 cells were treated as above, and medium were changed to LSM 16 prior to incubation with 5 μg/mL Dylight-488-labeled LDL for 3 hours. D. Representative fluorescence images. Scale bar: 10 μm. E. Quantitative measurement of LDL uptake. Results are from 3 independent experiments in triplicates. N = 9; *: p<0.01; ***: p<0.001.