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. 2020 May 21;15(5):e0233390. doi: 10.1371/journal.pone.0233390

Fig 4. Effects of IRF7 expression on promoter activity of murine MCP-1.

Fig 4

A: The promoter −2933nt to +71nt of the mouse MCP-1, upstream of the transcription start site, was inserted into the pGL4.19 reporter vector. Putative binding motifs are shown. B: The activity of MCP-1 promoter was measured in HEK293 cells with various lengths of 5’-fragments. Results are shown as fold inductions relative to the values in the cells transfected with Empty-pcDNA vector (n = 4). C: Luciferase assay performed in HEK 293 cells with MCP-1 promoter- pGL4.19 vector carrying one or two proximal ISRE mutations. Results are shown as fold inductions relative to the values in the cells transfected with Empty-pcDNA vector (n = 4). *p < 0.05 and **p < 0.01. All values are means ± SEM.