Fig 5. ChIP-PCR analysis of IRF7-myc-C binding to the mouse MCP-1 promoter.

A: Quantitative, real-time PCR was performed with the indicated primer pair using an anti-myc antibody immunoprecipitated DNA fragment as template. Normal mouse IgG was used as negative control (n = 4). B: PCR products were separated by agarose gel electrophoresis and detected by ethidium bromide staining. C: DNA and nuclear protein interaction was examined by EMSA. DNA probes for ISRE (−228 to −204 nt) (WT) and its mutant (Mut) and for ISRE (−178 to −154 nt) and its mutant were used. The DNA probes confirmed to bind to IRF7 in the past [14] was used as positive control (PC). The arrowhead indicates protein-DNA complex. *p < 0.05 and **p < 0.01. All values represent mean ± SEM.