Skip to main content
. 2020 May 21;15(5):e0222373. doi: 10.1371/journal.pone.0222373

Fig 4. Effect of the knockdown of PUM1 and PUM2 during monolayer cardiac differentiation.

Fig 4

(A) Scheme of the protocol used for the transduction and monolayer cardiomyogenic differentiation of hESCs. (B) Representative immunofluorescence images of DAPI, eGFP/NKX2.5 and cTnT staining in the population of transduced hESCs after 15 days of monolayer cardiac differentiation. Scale bars: 500 μm. (C-E) The DAPI+ (C), eGFP/NKX2.5+ (D) and cTnT+ (E) stained areas were determined using Operetta CLS and Analysis Software 4.5 (Perkin Elmer) through a sequence analysis of 21 images (5X objective) obtained in triplicate after 15 days of monolayer cardiac differentiation (n = 3). (F) Number of contractions/minute during cardiac monolayer differentiation (n = 3). *p<0.05, **p<0.01.