Fig 2. Cigarette smoke increases uptake of poly I:C.

Primary human SAEC were exposed to either air or cigarette smoke for 60 minutes at the air-liquid interface. Following exposure cells were treated with 2.5μg/mL of fluorescent poly I:C for the indicated times. (A) Time course analysis of fluorescence at 2, 6, and 19 hours post poly I:C treatment. (B) Representative fluorescent images of poly I:C positive cells taken 6 hours post poly I:C treatment. (C) Analysis of cell fluorescence by flow cytometry 6 hours post treatment with poly I:C. The experiment was repeated using SAEC derived from 3 separate donors and each pair of points represents an SAEC strain from a different donor.