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. 2020 May 21;15(5):e0232679. doi: 10.1371/journal.pone.0232679

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© 2020 Donovan et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — A) The N-terminal domain of Filaggrin 2 binds to SASPase 28. SPR sensorgram shows the binding between recombinant GST-Flag-SASPase 28 and MBP-HA FLG2 S100 (aa 2–95). MBP-HA FLG2 S100 (aa 2–95) was injected at 6 different concentrations (1, 2, 3, 4, 5 & 6 μM) across 1 μM of SASPase 28 captured by the GST antibody immobilized on a CM5 sensorship. B) The binding of SASPase 28 to the N terminal domain of Filaggrin 2 is specific. A goat polyclonal anti-GST was immobilized on a CM5 sensorchip to capture 1 μM of GST-Flag-SASPase 28 kDa and GST, respectively. The S100 proteins MBP-HA FLG2 S100 (aa 2–95), MBP-HA FLG S100 (aa 2–95) MBP-HA Hornerin S100 (aa 4–95) and MBP-HA TCHH S100 (aa 2–95) and the control MBP-HA were injected at a concentration of 3 and 6 μM respectively.