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. 2020 May 21;15(5):e0232679. doi: 10.1371/journal.pone.0232679

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© 2020 Donovan et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — An in vitro enzymatic assay using recombinant proteins of 28 kDa SASPase (0.5 μM), and FLG2Nter (aa 2–213) and FLG2Nter (aa 81–213) at different mass ratios to SASPase 28 in the presence of a fluorescent-labeled peptide Dabcyl-QIDRIMEK-Glu(Edans)-NH2 (0.1 mM). The histogram presents the mean values (+/-SD) of each assay performed in triplicate.