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. 2020 May 21;15(5):e0232679. doi: 10.1371/journal.pone.0232679

Fig 6. The N-terminal domain of Filaggrin 2 enhances the auto-activation of 28 kDa SASPase to its active 14 kDa form.

Fig 6

Recombinant SASPase 28 was incubated from 0 to 6 hours in the presence of equimolar amounts of recombinant protein FLG2Nter (aa 2–213). The auto-processing of 28 kDa SASPase into its catalytic 14 kDa form was analyzed by Western blot analysis using a monoclonal antibody that detects SASPase. A cropped image from S5 Fig shows the 14 kDa catalytic of SASPase. (B) semi quantitative analysis of the SASPase 14 band in the presence of FLG2Nter (green bars) as compared to SASPase 28 alone (red bars).