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. 2020 May 11;16(5):e1008797. doi: 10.1371/journal.pgen.1008797

Fig 5. UV-B impairs PIF4 binding to shade marker genes’ promoters in a UVR8-dependent manner.

Fig 5

(A) Schematic representation of PIL1 and XTR7 with G-boxes and regions amplified in ChIP-qPCR indicated. (B, C) PIF4-HA chromatin association in Col/ProPIF4:PIF4-3xHA (PIF4-HA) and uvr8-6/ProPIF4:PIF4-3xHA (uvr8/PIF4-HA). Ten-day-old seedlings were grown in long-day conditions under white light and exposed to low R:FR with (+) or without (-) 3-h supplemental narrowband UV-B at ZT3. ChIP-qPCR was performed for (B) PIL1 and (C) XTR7 promoters. ChIP data are presented as the percentage recovered from the total input DNA (% Input). Data shown are representative of three independent biological replicates (see S5A–S5D Fig). Error bars represent SD of three technical replicates. Immunoprecipitated DNA was quantified by qPCR using primers in the promoter regions containing G-boxes (region 1, ProPIL1_-1417; region 3, ProXTR7_-197) or control regions without G-boxes (region 2, ProPIL1_+1816; region 4, ProXTR7_-664).