Figure 2.

Quiescent IBD is associated with reduced numbers of Trm and γδ T cells in colonic tissue. A: Numbers and percentages of CD8⁺ γδ TCR^- CD103⁺ Trm recovered from IEL [upper graphs] and γδ CD103⁺ T cells in IEL [lower graphs], alongside CD39 and CD73 expression on these populations. B: Numbers and percentages of CD4⁺ CD103⁺ Trm [upper graphs] and CD8⁺ CD103⁺ Trm [lower graphs] recovered from LPL, alongside CD39 and CD73 expression. Full gating strategies are shown in supplementary methods. C: Total live cell numbers in IEL and LPL fractions [including epithelial cells]. D: Correlation of CD8 Trm and γδ T cell numbers in IEL populations from right colon biopsies of all donors. HC: healthy controls, n = 25; CD: Crohn’s disease, n = 12; UC: ulcerative colitis, n = 20. Median values ± 95% confidence intervals are shown; statistically significant differences between groups [Kruskal–Wallis test] are indicated. Spearman correlation coefficient was calculated in D. IEL, intraepithelial lymphocytes; LPL, lamina propria lymphocytes; IBD, inflammatory bowel disease.