Table 2. Cryo-EM data collection, refinement and validation statistics of CALHM6 and CALHM2.
| Dataset 3 CALHM6_Ca2+ |
Dataset 4 CALHM2_Ca2+ |
||
|---|---|---|---|
| 10-mer | 11-mer | ||
| Data collection and processing | |||
| Microscope | FEI Tecnai G2 Polara | FEI Tecnai G2 Polara | |
| Camera | Gatan K2 Summit + GIF | Gatan K2 Summit + GIF | |
| Magnification | 37,313 | 37,313 | |
| Voltage (kV) | 300 | 300 | |
| Electron exposure (e–/Å2) | 40 | 55 | |
| Defocus range (μm) | -0.8 to -3.0 | -0.8 to -3.0 | |
| Pixel size (Å) | 1.34 | 1.34 | |
| Initial particle images (no.) | 216,859 | 417,612 | |
| Final particle images (no.) | 98,104 | 63,310 | N/A |
| Reconstruction strategy1* | Std | Std | Std |
| Symmetry imposed | C10 | C11 | C1 |
| Global map resolution (Å) FSC threshold 0.143 |
4.39 | 6.23 | N/A |
| Map resolution range (Å) | 4.3-5.1 | 5.0-7.0 | N/A |
| Map sharpening B factor (Å2) | -259 | -435 | N/A |
| EMDB identifier | 10924 | 10925 | N/A |
| Refinement | N/A | ||
| Model resolution (Å) FSC threshold 0.5 |
4.4 | 6.6 | |
| Model composition Non-hydrogen atoms Protein residues |
19,560 2,520 |
21,516 2,772 |
|
|
B factors (Å2) Protein |
85 |
86 |
|
| R.m.s. deviations Bond lengths (Å) Bond angles (°) |
0.004 0.773 |
0.004 0.822 |
|
| Validation MolProbity score Clash score Poor rotamers (%) |
2.14 16.18 0 |
2.15 17.75 0 |
|
| Ramachandran plot Favored (%) Allowed (%) Disallowed (%) |
93.55 6.05 0.40 |
93.95 5.65 0.40 |
|
| PDB identifier | 6YTV | 6YTX | |
*Std – standard reconstruction; Loc – localized reconstruction.