Fig. 2.

Overexpression of N. benthamiana Nbwo causes dwarfism. (a) Phenotypes of Nbwo-overexpressing (-OE) transgenic plants. Compared to wild-type (WT) plants, the density of the trichomes in the stems and leaves of the transgenic lines decreased. (b) Relative expression levels of Nbwo and NbCycB2 in the transgenic lines as measured by qRT-PCR. Compared with the wild-type plants, the expression level of NbCycB2 decreased with the increase in total expression of Nbwo in the transgenic plants. Data are means (±SD), n=3. Expression is relative to that of the WT, the value of which was set as 1. (c) Root lengths in 2-week-old seedlings of the WT, NbCycB2-OE #1 T₁, Nbwo-OE #3 T₁, and Nbwo-RNAi #2 T₁. The scale bar is 1 cm. (d) Root hairs of 2-week-old seedlings of Nbwo-OE #3 T₁ and NbWo^V-OE #1 T₁. The root hairs were normal in the Nbwo-OE line. The scale bars are 1 mm. (e, f) SEM images of 10-d-old seedlings of (e) Nbwo-OE #3 T₁ and (f) NbWo^V-OE #1 T₁. The trichome density of Nbwo-OE plants was normal, whilst the NbWo^V-OE plants showed a significant increase. The scale bars are 500 μm. (This figure is available in colour at JXB online.)