Fig. 3.
Nicotiana benthamiana Nbwo and NbWoV can bind to the NbCycB2 promoter in vitro and in vivo. (a) Fragments of the NbCycB2 promoter were used for ChIP (P1–P3) and yeast one-hybrid (Y1H) assays (A–E). The numbers indicate the positions of the truncations. (b) The ratio of bound promoter fragments versus total input detected by qRT-PCR after immuno-precipitation of HA-NbWoV by HA antibodies. Data are means (±SE), n=3. (c) Y1H assays to determine the interactions of the NbCycB2 promoter fragments and AD-Nbwo or the negative control (AD) in the Y187 yeast strain. (d) Schematic diagram of the effector and reporter constructs used in the LUC assays. (e) Relative reporter activities in N. benthamiana protoplasts after transient transformation of the effector and reporter constructs. The relative LUC activity normalized to REN activity are shown (LUC/REN). Data are means (±SD), n=3. Significant differences were determined using Student’s t-test: **P<0.01. (This figure is available in colour at JXB online.)