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. 2020 Jan 28;71(6):1815–1827. doi: 10.1093/jxb/erz542

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© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Nicotiana benthamiana NbCycB2 can suppress the function of Nbwo, but has no effect on NbWo^V. (a) Co-expression of NbCycB2 in leaves of proNbCycB2-GFP-GUS transgenic plants inhibits the expression of GUS induced by overexpression of Nbwo, but it has no effect on the expression of GUS induced by NbWo^V. All the constructs were expressed under the control of the 35S promoter. The areas injected with P35S::Nbwo, P35S::NbWo^V, and P35S::NbCycB2 GV3101 strain are indicated. At 72 h after injection, the leaves were stained with GUS substrate. (b) Dual-LUC activity test, confirming that co-expression with NbCycB2 decreased the transactivation activity of Nbwo compared with NbWo^V. Data are means (±SD), n=3. Significant differences were determined using Student’s t-test: **P<0.01.