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. 2020 Jan 28;71(6):1815–1827. doi: 10.1093/jxb/erz542

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© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — Nicotiana benthamiana Nbwo and NbWo^V can bind to their own genomic DNA sequences. (a) The fragments of the Nbwo genomic sequences used in the ChIP (T1–T7) and yeast one-hybrid (Y1H) assays (G1–G4). (b) Ratio of bound genomic fragments versus total input detected by real-time PCR after immuno-precipitation from the NbWo^V-overexpressing (-OE) lines by HA antibodies. Data means (±SE), n=3. (c) Y1H assays to determine the interaction of Nbwo genomic sequence fragment bait constructs and AD-Nbwo, AD-NbWo^V, or empty-vector pGADT7 constructs in the Y187 yeast strain. The clones were grown on SD/–Leu/–His/–Trp (–L–W–H) with 60 mM 3-AT medium. (This figure is available in colour at JXB online.)