Fig. 10.

Identification of sites in the SOS1 sequence regulated by the CIPK8–CBL10 complex. Site-specific mutations in SOS1 were generated at the 1136th and 1138th serine residues as described in ‘Materials and methods’. The wild-type and mutant SOS1 genes (wild-type gene (DSPS), mutant S1136A (DAPS), mutant S1138A (DSPA), and double mutant S1136A/S1138A (DAPA)) were cloned into the vector p416, and transformed into AXT3K either lacking or co-expressing CIPK8 and CBL10. Transgenic and untransformed yeast cells were spotted on AP plates with or without NaCl as described in ‘Materials and methods’, and cultured at 28 °C for 3–5 d.