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. 2019 Dec 10;71(6):1801–1814. doi: 10.1093/jxb/erz549

Fig. 5.

Fig. 5.

CIPK8 complements salt sensitivity of cipk8 and cipk8sos2. A 5502 bp DNA fragment containing the promoter and coding region of CIPK8 (CIPK8P:CIPK8) was introduced into cipk8 or sos2cipk8 mutants through mediation of the vector pCAMBIA1300. The T3 generations of 10 transgenic homozygote lines of CIPK8 in cipk8 (CIPK8P:CIPK8/cipk8) and eight transgenic homozygote lines of CIPK8 in sos2cipk8 (CIPK8P:CIPK8/sos2cipk8) were used for screening. The transgenic lines, CIPK8P:CIPK8/cipk8-1, CIPK8P:CIPK8/cipk8-3, CIPK8P:CIPK8/sos2cipk8-4 and CIPK8P:CIPK8/sos2cipk8-6, were used for the following experiments. (A) CIPK8 expression levels in WT, cipk8, and CIPK8P:CIPK8/cipk8 lines were determined by RT-PCR with gene-specific primers (Supplementary Table S1). (B) WT, cipk8, and CIPK8P:CIPK8/cipk8 plants were grown in normal soil for 3 weeks, and then grown for 4 weeks in soil supplemented with 150 mM NaCl. Images were taken after 4 weeks of NaCl treatment and the fresh weights (C) were determined. (D) CIPK8 expression levels in WT, sos2, sos2cipk8, and CIPK8P:CIPK8/sos2cipk8 lines were determined by RT-PCR with gene-specific primers (Supplementary Table S1). (E) WT, sos2, sos2cipk8, and CIPK8P:CIPK8/sos2cipk8 plants were grown in normal soil for 3 weeks, and then grown for 4 weeks in soil supplemented with 150 mM NaCl. Images were taken after 4 weeks of NaCl treatment, and the fresh weights (F) were determined. Data represent the mean ±SE of nine replicates and letters above the columns indicate significant differences with P<0.05. (This figure is available in color at JXB online.)