Fig. 6.

Identifying interactions between CIPK8 and CBL proteins. (A) Interaction between CIPK8 and CBL proteins was assayed using a yeast two-hybrid method. Yeast cells harboring different fusion protein combinations were plated on DDO medium (lacking Leu and Trp) or QDO/X/A (lacking Leu, Ade, His, and Trp, but supplemented with 40 µg ml⁻¹ X-α-Gal and 125 ng ml⁻¹ aureobasidin A). The combination pGADT7-T and pGBKT7-53 was used as a positive control, and pGADT7-T and pGBKT7-lam was used as a negative control. (B) Interactions between CIPK8 with CBL10 determined using firefly luciferase complementation imaging assays in Nicotiana benthamiana leaves. The interaction between SOS2 and CBL10 was used as positive conterol (C). cLUC, C-terminal region of firely luciferase; nLUC, N-terminal region of firefly luciferase. Three independent experiments were carried out in this study. (This figure is available in color at JXB online.)