Fig. 7.

Effect of CsJAZ2 and CsICE2 interaction on CsJAZ5 inhibition of CsMYC2 and CsTSB2 expression. (A) Phylogenetic analysis of ICE homolog protein in different plants. The Neighbor–Joining tree was constructed with ClustalW (1000 bootstrap replications). (B) Yeast two-hybrid assay showing the interaction of CsJAZs and CsICEs. BD, pGBKT7; AD, pGADT7. (C) BiFC analysis showing the interaction of CsJAZ2 and CsICEs in the Arabidopsis mesophyll protoplast. From left to right: GFP channel; chlorophyll autofluorescence; and merged images. (D) Transient transactivation assays showing that CsTSB2 expression activation by CsMYC2a is modulated by CsJAZ2 and CsICE2 in the Arabidopsis myc2 mutant mesophyll protoplast. pCsTSB2:GUS was co-transformed with other constructs into Arabidopsis myc2 mutant mesophyll protoplasts. 35S:LUC was used as an internal control. Different letters indicate statistically significant differences (one-way ANOVA followed by Duncan’s multiple test, P≤0.05). All data are means of three independent experiments, and error bars represent ±SD. (This figure is available in color at JXB online.)