Table 1.
The effect of physiological saline or prazosin treatment on noradrenalline, GABA, glycine, glutamate levels and acetylcholine esterase activity in different brain regions of rats subjected to stress
| NTs (ng/mg tissue) | Brain regions | Stress (−) | Stress (＋) | Two-way ANOVA statistics (source of variation) | ||
|---|---|---|---|---|---|---|
|
|
|
|||||
| Physiological saline | Prazosin | Physiological saline | Prazosin | |||
| NA | AC | 3.07 ± 0.24 | 3.70 ± 0.30 | 3.88 ± 0.22** | 3.68 ± 0.40 | Stress (df = 1, f = 6.652, p < 0.05) Interaction (df = 1, f = 7.986, p < 0.05) |
| DH | 2.83 ± 0.13 | 2.88 ± 0.3 | 3.31 ± 0.19 | 2.80 ± 0.50 | Stress (df = 1, f = 1.058, p > 0.05) Treatment (df = 1, f = 1.401, p > 0.05) |
|
| PFC | 2.63 ± 0.4 | 3.1 ± 0.2 | 2.79 ± 0.60 | 2.85 ± 0.50 | Stress (df = 1, f = 0.02554, p > 0.05) Treatment (df = 1, f = 1.265, p > 0.05) |
|
| RP | 7.39 ± 0.23 | 3.77 ± 0.4 | 18.89 ± 3.36* | 3.50 ± 0.20 | Interaction (df = 1, f = 6.697, p < 0.05) | |
| GABA | AC | 3.07 ± 0.24 | 3.70 ± 0.22 | 3.89 ± 0.23* | 3.68 ± 0.18 | Interaction (df = 1, f = 4.447, p < 0.05) |
| DH | 122.90 ± 2.83 | 119.60 ± 17.10 | 126.30 ± 7.61 | 112.60 ± 13.60 | Stress (df = 1, f = 1.472, p > 0.05) Treatment (df = 1, f = 0.5429, p > 0.05) |
|
| PFC | 103.10 ± 10.60 | 124.70 ± 8.10 | 110.50 ± 11.60 | 113.60 ± 4.80 | Treatment (df = 1, f = 8.300, p < 0.01) Interaction (df = 1, f = 4.681, p < 0.05) |
|
| RP | 136.20 ± 6.58 | 157.40 ± 10.10 | 129.30 ± 11.10 | 135.10 ± 12.60 | Stress (df = 1, f = 4.258, p > 0.05) Treatment (df = 1, f = 3.683, p > 0.05) |
|
| Gly | AC | 4.03 ± 0.44 | 4.60 ± 1.20 | 4.64 ± 0.57 | 4.30 ± 0.90 | Stress (df = 1, f = 0.0953, p > 0.05) Treatment (df = 1, f = 0.0569, p >0.05) |
| DH | 5.08 ± 0.84 | 3.90 ± 1.30 | 4.67 ± 0.61 | 4.60 ± 0.60 | Stress (df = 1, f = 0.0802, p > 0.05) Treatment (df = 1, f = 0.9751, p > 0.05) |
|
| PFC | 4.53 ± 1.00 | 4.15 ± 1.10 | 3.99 ± 0.70 | 4.44 ± 0.40 | Stress (df = 1, f = 0.0992, p > 0.05) Treatment (df = 1, f = 0.0058, p > 0.05) |
|
| RP | 4.26 ± 0.50 | 3.30 ± 0.40 | 4.22 ± 0.38 | 4.00 ± 0.80 | Stress (df = 1, f = 0.6570, p > 0.05) Treatment (df = 1, f = 2.176, p > 0.05) |
|
| Glu | AC | 32.22 ± 2.79 | 36.00 ± 4.80 | 31.81 ± 2.28 | 38.60 ± 8.50 | Stress (df = 1, f = 0.1402, p > 0.05) Treatment (df = 1, f = 3.263, p > 0.05) |
| DH | 31.30 ± 2.57 | 40.20 ± 10.10 | 32.91 ± 3.05 | 33.40 ± 6.20 | Stress (df = 1, f = 0.4574, p > 0.05) Treatment (df = 1, f = 0.1865, p > 0.05) |
|
| PFC | 35.07 ± 8.40 | 45.25 ± 7.90 | 35.08 ± 7.20 | 36.48 ± 8.50 | Stress (df = 1, f = 0.1309, p > 0.05) Treatment (df = 1, f = 2.291, p >0.05) |
|
| RP | 31.45 ± 1.99 | 37.00 ± 4.60 | 36.14 ± 2.29 | 40.80 ± 12.10 | Stress (df = 1, f = 0.2541, p > 0.05) Treatment (df = 1, f = 0.1633, p > 0.05) |
|
| AChE A | AC | 2.76 ± 0.31 | 185.70 ± 62.00 | 31.49 ± 8.36 | 145.10 ± 62.00 | Treatment (df = 1, f = 9.948, p < 0.05) |
| DH | 29.76 ± 19.07 | 255.00 ± 50.00* | 3.87 ± 1.00 | 174.00 ± 50.00 | Stress (df = 1, f = 3.693, p > 0.05) Treatment (df = 1, f = 44.84, p > 0.05) Interaction (df = 1, f = 3.066, p > 0.05) |
|
| PFC | 107.50 ± 16.10 | 847.80 ± 69.10 | 548.6 ± 98.80 | 648.03 ± 76.80 | Treatment (df = 1, f = 6.803, p < 0.05) | |
| RP | 1.53 ± 0.22 | 39.10 ± 10.00 | 92.23 ± 23.75 | 175.00 ± 74.00* | Stress (df = 1, f = 9.134, p > 0.05) Treatment (df = 1, f = 2.578, p > 0.05) Interaction (df = 1, f = 0.3628, p > 0.05) |
|
Values are presented as mean ± standard error of the mean.
GABA, gamma aminobutyric acid; NTs, neurotransmitters; NA, noradrenalline; Gly, glycine; Glu, glutamate; AChE A, acetylcholine esterase activity; AC, amygdaloid complex; DH, dorsal hippocampus; PFC, prefrontal cortex; RP, rostral pons.
*
p < 0.05, Bonferroni post-test, significantly different from others. **p < 0.01, Bonferroni post-test, significantly different from others.