Figure 1.

Overview of the high-speed quantitative reverse transcription polymerase chain reaction (RT-qPCR) system. (a) GeneSoC equipment connected to two sets of PCR units on the right side. Up to four PCR units can be connected and one microfluidic chip can be loaded on each PCR unit. (b) Disposable microfluidic chip for high-speed RT-qPCR using the GeneSoC system. (c) Schematic of liquid flow on a disposable microfluidic chip. White circle with star, inlet for master mix for RT-qPCR; grey circle with asterisk, injection port for each microblower for the high-speed shuttle of the PCR solution; Heater 1, incubation for the RT reaction; Heaters 2 and 3, heaters for thermal cycling. The PCR solution cycles at high speed between Heaters 1 and 2.