. 2020 May 22;105(4):615–618. doi: 10.1016/j.jhin.2020.05.025

Table I.

Components of the reaction mixtures for high-speed quantitative reverse transcription polymerase chain reaction (RT-qPCR)

Reagents	Final concentration
2× OneStep RT-PCR Buffer III^a	1×
Primer (forward)	2.4 μM, 5′-AAATTTTGGGGACCAGGAAC-3′
Primer (reverse)	3.2 μM, 5′-TGGCAGCTGTGTAGGTCAAC-3′
Probe	0.4 μM, FAM-ATGTCGCGCATTGGCATGGA-TAMRA
10 x ROX	0.2×
PrimeScript RT enzyme Mix II^a	2 U/μL
SpeedSTAR HS DNA Polymerase	0.25 U/μL

Each reagent was included in the One Step PrimeScript RT-PCR Kit (Qiagen, Hilden, Germany).