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. 2020 Apr 17;28(2):195–207. doi: 10.1007/s10577-020-09632-3

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© The Author(s) 2020

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Fig. 4 — DSB formation does not seem to be affected by temperature. Focus counts of immune-detected Rec7-GFP and Rad11-GFP on Hop1-positive nuclear spreads from meiotic, azygotic timecourses at timepoints with maximum horsetail (meiotic prophase I) nucleus frequency; black horizontal lines indicate mean values, error bars represent standard deviation; for details of data see Table S6. a Number of Rec7-GFP foci per nucleus at 16 °C (25 h timepoint, n = 36) and 33 °C (5 h timepoint, n = 35) from meiotic timecourses of UoA825. b Examples of chromatin spreads evaluated in (a), Rec7-GFP in green, Hop1-13myc in red and DNA stained by Hoechst 33342 in blue; scale bar represents 5 μm. c Number of Rad11-GFP foci per nucleus at 16 °C (25 h timepoint, n = 35) and 33 °C (5 h timepoint, n = 35) from meiotic timecourses of UoA826. d Examples of chromatin spreads evaluated in (c), Rad11-GFP in green, Hop1-13myc in red and DNA stained by Hoechst 33342 in blue; scale bar represents 5 μm