Skip to main content
. 2020 May 21;11(5):387. doi: 10.1038/s41419-020-2591-0

Fig. 3. Lycorine significantly elevates SAV1 protein levels through degradation of the protein via ubiquitination-lysosome system.

Fig. 3

After A549 and SPC-A-1 cells were treated with lycorine (LH) at the concentrations of 0–10 μM for 72 h, the protein levels of SAV1, total MST1 (T-MST1), and phosphorylated MST1 (p-MST1) were determined by Western blotting. SAV1, p-MST1 protein levels were increased in lung cancer SPC-A-1 (a, b) and A549 (c, d) cells by lycorine in a dosage-dependent manner. Treatment of the lung cancer cells with the ubiquitin inhibitor MG132 significantly increased SAV1 protein levels, combination of lycorine with MG132 further increased SAV1 protein levels (e, f). Co-IP and western blotting analysis displayed that lycorine significantly decreased SAV1 protein ubiquitination (g, h). Data are shown as mean ± SD of three independent replicates.