Table 3.
Number of genes and miRNAs with expression values correlated to mineral amount, per mineral and per matching attribute.
| Minerals | DEGsa | Significant RIFb | TFsc | cis eQTLsd | trans eQTLse | miRNAsf | No attributesg |
|---|---|---|---|---|---|---|---|
| Ca | 0 | 3 | 2 | 0 | 3 | 5 | 14 |
| Cu | 1 | 4 | 1 | 0 | 1 | 5 | 28 |
| K | 2 | 5 | 2 | 0 | 7 | 3 | 19 |
| Mg | 2 | 6 | 2 | 0 | 5 | 6 | 23 |
| Na | 3 | 7 | 2 | 0 | 13 | 6 | 21 |
| P | 0 | 1 | 2 | 0 | 3 | 6 | 12 |
| S | 1 | 8 | 3 | 0 | 8 | 6 | 34 |
| Se | 1 | 9 | 2 | 1 | 3 | 6 | 17 |
| Zn | 0 | 6 | 1 | 0 | 3 | 9 | 27 |
| Fe | 3 | 19 | 0 | 0 | 2 | 5 | 9 |
The numbers result from both PCIT analysis: PCIT general, with genomic estimates of breeding values (GEBVs) for mineral, genes and miRNAs expression, and PCIT miRNA, with only mineral GEBVs and miRNAs expression. Mineral amount, normalized RNAseq obtained gene and miRNA expression levels were from Nelore steers’ Longissimus thoracis muscle. Columns represent the number of matches with attributes used for this analysis. aDifferentially expressed genes described in refs. 9,10. bGenes and miRNAs with significant regulatory impact factor in the present work. cTranscription factors17. dGenes affected by cis eQTLs18. eGenes affected by trans eQTLs18. fMicro RNAs. gGenes and miRNAs with expression values correlated to each mineral that were not identified in previous works.