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. 2020 May 21;10:8418. doi: 10.1038/s41598-020-65423-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reduction of renal expression and activity of Sirt3 paralleled with an increase of ROS production in diabetic mice. (a) qRT-PCR analysis of Sirt3 mRNA levels at 14 weeks of age in kidney of BTBR wild-type (WT) mice (n = 9) and BTBR ob/ob mice treated with vehicle (n = 6) or honokiol (n = 9) from 8 weeks of age. (b) Representative images and quantification of glomerular acetylated SOD2 staining in BTBR WT mice (n = 9) and in BTBR ob/ob mice treated with vehicle (n = 6) or honokiol (n = 9) at 14 weeks of age. Insets show acetylated SOD2 in podocytes of WT and diabetic mice (arrowheads). Scale bars: 20 μm. (c) Representative images and quantification of glomerular nitrotyrosine staining in BTBR WT mice (n = 9) and in BTBR ob/ob mice treated with vehicle (n = 6) or honokiol (n = 9) at 14 weeks of age. Insets show nitrotyrosine staining in podocytes of WT and diabetic mice (arrowheads). Scale bars: 20 μm. (d,e) qRT-PCR analysis of Sirt1 (d), and Sirt6 (e) mRNA levels in kidney of BTBR WT mice (n = 9) and BTBR ob/ob mice treated with vehicle (n = 6) or honokiol (n = 9) at 14 weeks of age. Data are mean ± SEM and were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test, *p < 0.05, ***p < 0.001 vs BTBR WT mice; ^#p < 0.05, ^###p < 0.001 vs BTBR ob/ob + vehicle.