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. 2020 Feb 19;14(6):1384–1395. doi: 10.1038/s41396-020-0616-4

Fig. 3. Protein of an ancestral HTG frr in leafhoppers is specifically expressed in Sulcia-bearing bacteriocytes in ALF.

Fig. 3

a Bayesian phylogeny of the horizontally transferred bacterial ribosomal recycling gene (frr; see methods and supplementary Fig. S1 for full phylogeny description). b Comparison of protein expression of frr between ALF bacteriome and body tissue using immunoblot approach. c Immunofluorescence localization of frr in Sulcia-ALF bacteriocytes with anti-frr primary antibody and Alexa Fluor 555 secondary antibody (red). DNA is counter-stained with DAPI (blue). The upper and lower dashed lines show the boundary between Sulcia and Nasuia bacteriocytes. White arrows indicate the nuclei of Nasuia-containing bacteriocytes. Scale bar is 5 µm (color figure online).