Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 21;10:8414. doi: 10.1038/s41598-020-65488-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

CD8⁺ TILs express high levels of cytotoxic molecules and PD-1. (a) Relative mRNA expression of IFN-γ, IL-17A and IL-10 in CD4⁺ and CD8⁺ T cells isolated from PB and tumour tissue. Blood was obtained at days 0, 14 and 28, and the tumour was resected at day 14 after tumour transplantation. The mRNA levels were assessed by real-time PCR and are presented relative to β-actin levels. (b) Intracellular staining for IFN-γ and IL-17A in CD8⁺ T cells from PB and tumours. Blood and the tumour were obtained at day 14. (c) Relative mRNA expression of perforin, granzyme B and PD-1 in CD8⁺ T cells isolated from PB and tumour tissue. The mRNA levels were assessed as in (a). (d) Flow cytometric analysis of the expression of granzyme B and PD-1 in CD8⁺ T cells and CD8⁺ T_EM cells from tumours.