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. 2020 May 21;10:8414. doi: 10.1038/s41598-020-65488-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CXCR3 and its ligands are upregulated in the tumour microenvironment. (a) Relative mRNA expression of chemokine receptors in CD4⁺ and CD8⁺ T cells isolated from PB and tumour tissue. The mRNA levels were assessed by real-time PCR and are presented relative to β-actin levels. (b) Flow cytometric analysis of CXCR3 expression in CD8⁺ T cells and CD8⁺ T_EM cells from tumours. (c) Relative expression of chemokines in cultured PTY cells, skin and tumour. The mRNA levels were assessed as in (a).