Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 21;11:2549. doi: 10.1038/s41467-020-16312-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Mitochondrial morphology outlined by Tom20 antibodies in control and PGAM5^−/− ARPE-19 cells. Scale bar = 20 µm. n = 5. b Western blots showing upregulation of phosphor-Drp1(S637) but not total Drp1 in PGAM5^−/− ARPE-19 cells. α-Tubulin was used as loading control. n = 4. c Western blots showing PGAM5 cleavage and Drp1(S637) dephosphorylation in ARPE-19 cells by CCCP treatment. n = 3. d Co-immunoprecipitation experiment using Axin1 antibody, showing that Axin1 interacts with both Drp1 and cleaved PGAM5 in ARPE-19 cells. n = 3. e Western blots showing upregulation of mitochondrial proteins (Tom20, CYTC, CYPD) and downregulation of PGC1α in PGAM5^−/− ARPE-19 cells. α-Tubulin was used as loading control. n = 4. f Increased mitochondrial DNA in PGAM5^−/− ARPE-19 cells. n = 5. *p = 0.0111, two-tailed unpaired t-tests; error bars, mean ± s.e.m. g Increased mitochondrial protein Cypd in the RPE/choroid of Pgam5^−/− mice. α-Tubulin was used as loading control. n = 3. h Decreased mitochondrial turnover in PGAM5^−/− ARPE-19 by MitoTimer transfection and labeling. Scale Bar equals to 20 µm. n = 3. i Mitochondrial membrane potential change as labeled by JC-1 in WT and PGAM5^−/− ARPE-19 cells. Scale bar = 20 µm. n = 5. j ATP level change as measured in short-term (1 week) and long-term (8 weeks) culture of WT and PGAM5^−/− ARPE-19 cells. n = 3, ****p < 0.0001, two-way ANOVA Tukey’s multiple comparisons test; error bars, mean ± s.e.m. k ROS change as measured in short-term (1 week) and long-term (8 weeks) culture of WT and PGAM5^−/− ARPE-19 cells. n = 3, *p < 0.05; ****p < 0.0001, two-way ANOVA Tukey’s multiple comparisons test. n.s. represents no significance. Error bars, mean ± s.e.m.; for assays in the figure, n represents the number of biologically independent experiments. Images were captured under same settings, and representative images were shown. Source data are available as a Source Data file.