Fig. 2. Verfication of the hydBA deletion in A. woodii ΔpyrEvia PCR and Western Blot analysis.

Agarose gel showing PCR products of the wild type (WT), pyrE (ΔpyrE) and hydBA mutant (ΔhydBA) using primers which anneal outside the hydBA region (a). Detection of HydB and HydA in crude extract of the wild type and hydBA mutant blotted on a nitrocellulose membrane and by using antibodies against HydB and HydA (b). M, Marker; NC, negative control; WT, A. woodii wild type.