Fig. 4. Off-line and off-gas data for stirred-tank reactor fermentations of A. woodii wild type.

The wild type was grown in complex medium with 20 mM fructose as carbon and energy source and the cultivation broth was sparged with 10 ml min⁻¹ 20 % CO₂ in nitrogen. The upper half shows the average off-line values for OD₆₀₀ (◆), concentration of fructose (■) and acetic acid (▲) of four cultivations whilst the bottom half shows off-gas flow rates for hydrogen (solid line) and carbon dioxide (dashed line). The grey areas in the bottom half depict the variation of the off-gas values.