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. 2020 May 21;10:8441. doi: 10.1038/s41598-020-64003-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Entropy plots. (A) The nucleotide variation amongst the 99 bp fragment of the AIV M gene segment (positions 25–124) amplified by protocols 1, 2 and 3 shown as entropy. (B) The nucleotide variation amongst the 182 bp fragment of the AIV M gene segment (position 77–259) amplified by protocol 4 shown as entropy. The height of each column is proportional to the nucleotide variation at the given position. In red the primers and probes binding regions.