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. 2020 May 21;10:8391. doi: 10.1038/s41598-020-64845-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Na⁺/K⁺ α1-subunit protein levels in neurons generated from healthy volunteers and ChAc patients. (A) Original histogram of Na⁺/K⁺ α1-subunit protein abundance determined by flow cytometry in neurons generated from healthy volunteers (red) and ChAc patients (blue). (B) Means ± SEM (n = 6-7) of Na⁺/K⁺ α1-subunit protein abundance (mean fluorescence intensity; MFI) determined by flow cytometry in neurons generated from healthy volunteers (black diamond, triangle, reverse triangle) and in neurons generated from ChAc patients (blue circle, square). Data was generated from two independent culture experiments. **(p < 0.01) indicates significant difference between two groups, unpaired t-test.