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. 2020 May 21;11(5):383. doi: 10.1038/s41419-020-2598-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Interleukin (IL)-1β and IL-18 mRNA expression in normal and hyperglycaemic HUVECs. b Western blot analysis of NLRP3 inflammasome expression in normal and hyperglycaemic HUVECs. c The mRNA expression of NLRP3 inflammasome in normal and hyperglycaemic HUVECs. d Western blot analysis of MARK4 expression in normal and hyperglycaemic HUVECs. e The mRNA expression of MARK4 in normal and hyperglycaemic HUVECs. f The effects of siMARK4 on high glucose-induced NLRP3 inflammasome protein expression in hyperglycaemic HUVECs. g The effects of siMARK4 on high glucose-induced NLRP3 inflammasome mRNA expression in hyperglycaemic HUVECs. h The effects of siMARK4 on high glucose-induced IL-1β and IL-18 mRNA expression in hyperglycaemic HUVECs. (*P ≤ 0.001, **P ≤ 0.0001, compared with the control group; ^#P ≤ 0.001, ^##P ≤ 0.0001, compared with the high glucose treatment group, n = 5/group).