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. 2020 May 21;10:8425. doi: 10.1038/s41598-020-65339-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sox9 expression in muscle. (a–d) Head at E10 and (e-h) limb at E10 and E12. All panels show immunohistochemical staining of desmin (green) and Sox9 (red). (b, c) High-magnification view of a square in (a) and (g) high-magnification view of a square in (f). (d, h) Comparison of Sox9⁺ progenitor of CNCs with those of the CPM. (d) The mass composed of muscle progenitor cells has few Sox9⁺ progenitor cells compared to CNCs (P < 0.001). (h) The limb muscle has few Sox9+ progenitor cells compared to the future tendon and bone (P < 0.01). Sox9, SRY-box containing gene 9; CNC, neural crest cell; CPM, cranial paraxial mesoderm; MTJ, myotendinous junction.