FIGURE 5.

Gene expression analysis during phagocytosis. P. aeruginosa WT strain was used to infect THP1 macrophages (MOI 2.5) for 1 h. RNA from culture medium, corresponding to unphagocytosed bacteria, was extracted and used for comparison (dark line set to 1 on the figure). RNA from macrophages was then extracted 0.5, 2, and 6 h post-infection. Mean values and standard deviations (error bars) of three independent experiments are indicated.