TABLE 2.

Effect of cadA or cadR deletion on MTC.

	WT pME6001	ΔcadA pME6001	ΔcadA pME6001-cadA	ΔcadR pME6001	ΔcadR pME6001-cadR
ZnCl2	8	5	8	6	8
CdCl2	4	0.25	4	1	4
CoCl2	1	1	1	1	1
NiCl2	2	2	2	2	2
CuCl2	4	4	4	4	4
Pb(NO3)2	10	8	10	8	10

Maximum tolerable concentration of the WT, and of the ΔcadA and ΔcadR mutants carrying an empty plasmid, or a plasmid containing the mutated gene. MTCs are given in mM. Values represent the mean of three independent determinations. Metal concentrations in Supplementary Table S3.