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. 2020 May 15;11:913. doi: 10.3389/fmicb.2020.00913

TABLE 2.

Plasmid used and their features.

Plasmid Size (bp) Features Resistance Source
pMK-lcn2 2930 Encodes LCN2 Kan GeneArt synthesis (Invitrogen)
pMK-Ex-FABP 2860 Encodes Ex-FABP Kan GeneArt synthesis (Invitrogen)
pMK-Cal-γ 2881 Encodes Cal-γ Kan GeneArt synthesis (Invitrogen)
pMK-α1-glyco 2935 Encodes α1-glycoprotein Kan GeneArt synthesis (Invitrogen)
pET21a 5443 Requires T7 RNA polymerase and under LacUV5 control Amp Novagen
pET-lcn2 5904 pET21a vector used for LCN2 over-production Amp This study
pET-Ex-FABP 5907 pET21a vector used for Ex-FABP over-production Amp This study
pET-Cal-γ 5928 pET21a vector used for CAL-γ over-production Amp This study
pET-α1-glyco 5982 pET21a vector used for α-1-glycoprotein over-production Amp This study
pKD3 2804 λ red template vector Cm, Amp Datsenko and Wanner, 2000
pKD46 6329 T°C sensitive (30°C) λ red expressing vector encoding exo, beta and gam and under AraC control Amp Datsenko and Wanner, 2000
pCP20 9332 T°C sensitive (30°C) vector encoding FLP recombinase Cm, Amp H. Mori, Japan