TABLE 2.
Plasmid used and their features.
Plasmid | Size (bp) | Features | Resistance | Source |
pMK-lcn2 | 2930 | Encodes LCN2 | Kan | GeneArt synthesis (Invitrogen) |
pMK-Ex-FABP | 2860 | Encodes Ex-FABP | Kan | GeneArt synthesis (Invitrogen) |
pMK-Cal-γ | 2881 | Encodes Cal-γ | Kan | GeneArt synthesis (Invitrogen) |
pMK-α1-glyco | 2935 | Encodes α1-glycoprotein | Kan | GeneArt synthesis (Invitrogen) |
pET21a | 5443 | Requires T7 RNA polymerase and under LacUV5 control | Amp | Novagen |
pET-lcn2 | 5904 | pET21a vector used for LCN2 over-production | Amp | This study |
pET-Ex-FABP | 5907 | pET21a vector used for Ex-FABP over-production | Amp | This study |
pET-Cal-γ | 5928 | pET21a vector used for CAL-γ over-production | Amp | This study |
pET-α1-glyco | 5982 | pET21a vector used for α-1-glycoprotein over-production | Amp | This study |
pKD3 | 2804 | λ red template vector | Cm, Amp | Datsenko and Wanner, 2000 |
pKD46 | 6329 | T°C sensitive (30°C) λ red expressing vector encoding exo, beta and gam and under AraC control | Amp | Datsenko and Wanner, 2000 |
pCP20 | 9332 | T°C sensitive (30°C) vector encoding FLP recombinase | Cm, Amp | H. Mori, Japan |