TABLE 5.
Lipocalins-binding affinities towards apo- and ferric-ligands obtained using isothermal titration calorimetry (in TBS pH 7.4 at 30°C) in this study and compared values from the literature. Kd is the equilibrium dissociation constant, n the binding stoichiometry, ΔS the entropy, and ΔH the enthalpy.
| Protein | Ligands | Reference | Kd nM | n | ΔS cal/mol, K | ΔH cal/mol |
| LCN2 | apo-Ent | This study | 58.4 ± 12.5 | 0.872 | −58.9 | −2.78 × 104 |
| Abergel et al., 2006 | 3.57 | |||||
| Fe3+-Ent | This study | 11.8 ± 8.8 | 0.863 | −50.2 | −2.62 × 104 | |
| Goetz et al., 2002 | 0.41 | |||||
| Fischbach et al., 2006 | 0.43 | |||||
| Li et al., 2015 | 240 | |||||
| Ex-FABP | apo-Sal (or DGE) | This study | Under threshold | |||
| Fischbach et al., 2006 | Under threshold | |||||
| apo-Ent | This study | 86.2 ± 14.6 | 0.617 | −60.9 | −2.83 × 104 | |
| Correnti et al., 2011 | 0.5 ± 0.15 | |||||
| Fe3+-Ent | This study | 5.3 ± 3.8 | 0.672 | −26.1 | −1.94 × 104 | |
| Correnti et al., 2011 | 0.22 ± 0.06 | |||||
| apo-Sal (or DGE) | This study | Under threshold | ||||
| Correnti et al., 2011 | Under threshold | |||||
| Cal-γ | apo-Ent | This study | Under threshold | |||
| apo-Sal (or DGE) | This study | Under threshold | ||||
| α-1-ovoglycoprotein | apo-Ent | This study | Under threshold | |||
| apo-Sal (or DGE) | This study | Under threshold |